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KMID : 0903520040470040373
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2004 Volume.47 No. 4 p.373 ~ p.378
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Isolation, Identification and Optimal Culture Condition of Bacillus sp. FF-9 Having Antifungal Activity on the Turf Grass Pathogens Caused by Rhizoctonia solani AGII-II
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Park Jin-Chul
Yoo Ji-Hyun
Cha Jae-Young
Kim Min-Seok
Cho Young-Su
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Abstract
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In this study, established soil-borne Bacillus sp. FF-9 with strong antifungal activity was isolated for identification and to determine optimal culture condition. By using 16s rDNA sequencing method, FF-9 of the selected bacteria was identified as genus Bacillus sp.. Bacillus sp. FF-9 was cultured at 30oC, for 24 h in the LB medium. Cell growth increased quickly after 6 h and the highest cell growth was indicated at 12 h. The most antifungal activity against Rhizoctoina solani AGII-II appeared at 18 h and the optimal temperature and pH were 30 and pH 8.0, respectively. A testing of carbon and nitrogen sources showed the highest antifungal activity at 1% lactose and 1% yeast extract Furthermore an addition of salt showed the most antibiotic activity in the 0.15% K2HPO4.
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KEYWORD
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antifungal activity, Bacillus sp., Rhizoctonia solani AGII-II
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